UV-C irradiation is highly effective in inactivating and inhibiting SARS-CoV-2 replication
Publication date: 08/06/2020 – E&P Code: repo.epiprev.it/1750
Authors: Andrea Bianco1, Mara Biasin2, Giovanni Pareschi1, Adalberto Cavalieri3, Claudia Cavatorta3, Claudio Fenizia2, Paola Galli1, Luigi Lessio4 , Manuela Lualdi3, Edoardo Redaelli1, Irma Saulle2,5, Daria Trabattoni2, Alessio Zanutta1, Mario Clerici5,6
Abstract: The potential virucidal effects of UV-C irradiation on SARS-CoV-2 were experimentally evaluated for different illumination doses and virus concentrations (1000, 5, 0.05 MOI). Both virus inactivation and replication inhibition were investigated as a function of these parameters. At a virus density comparable to that observed in SARS-CoV-2 infection, an UV-C dose of just 3.7 mJ/cm2 was sufficient to achieve a 3 log inactivation, and complete inhibition of all viral concentrations was observed with 16.9 mJ/cm2. These results could explain the epidemiological trends of COVID-19 and are important for the development of novel sterilizing methods to contain SARS-CoV-2 infection.
Cite as: Andrea Bianco, Mara Biasin, Giovanni Pareschi et. al. (2020). UV-C irradiation is highly effective in inactivating and inhibiting SARS-CoV-2 replication. E&P Repository https://repo.epiprev.it/1750
Topic: COVID-19
Key words: COVID-19, inibizione replicazione virus, SARS-CoV-2, UV, virus,
AVVERTENZA. GLI ARTICOLI PRESENTI NEL REPOSITORY NON SONO SOTTOPOSTI A PEER REVIEW.
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Affiliations:
1 Italian National Institute for Astrophysics (INAF) – Brera Astronomical Observatory, Milano/Merate, Italy
2 Department of Biomedical and Clinical Sciences L. Sacco, University of Milano, Milano, Italy
3 Department of Imaging Diagnostic and Radioterapy, IRCCS Foundation, Istituto Nazionale dei Tumori, Milano, Italy
4 Italian National Institute for Astrophysics (INAF) – Padova Astronomical Observatory, Padova, Italy
5 Department of Pathophysiology and Transplantation, University of Milano, Milano, Italy. (6) Don Gnocchi Foundation, IRCCS Foundation, Milano, Italy.
Authors’ contributions: Equal contribution by all authors.
Competing interests: No conflicts of interest.
Funding disclosure: This study has been conducted only with institutional funds and supports by INAF and University of Milano.
Ethics committee approval: The study was based on publicly available aggregate data. No Ethics committee approval was necessary.
Copyright: Il detentore del copyright è l’autore/finanziatore, che ha concesso a “E&P Repository” una licenza per rendere pubblico questo preprint. The copyright holder for this preprint is the author/funder, who has granted E&P Repository a license to display the preprint in perpetuity.
Terms of distribution: CC BY
Figure 1. Viral replication of UV-irradiated SARSTCoV-2 virus in in vitro VeroE6 cells. Vero E6 cells were infected with UV-C irradiated SARSTCoV-2 virus at a MOI of 0.05 (A, B and C), 5 (D, E and F) or 1000 (G, H and I).
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